Transgenic Core

When it comes to using a mouse or rat genome to study human disease, you need the best experimental model available to advance your research and propel discovery. That’s where we come in. The Transgenic Core at Children’s Hospital Research Institute can build you complex mouse or rat models, genetically manipulating the mouse or rat genome to meet your specific research needs. This is accomplished by using cutting-edge and classical genetic engineering approaches. We have successfully created over 40 mouse lines using the CRISPR system, and more recently created our first CRISPR Knockout rat line.

Our core features state-of-the-art services, including:

• CRISPR Cas9 mRNA microinjection and Cas9 RNP electroporation
• DNA construct microinjection
• Embryonic Stem (ES) cell microinjection
• Strain rederivation and rescue
• Embryo and sperm cryopreservation, importation and exportation

The Transgenic Core is a service sponsored by the CHOP Research Institution to enable investigators to drive cutting-edge basic and bench-to-bedside research. The mission of the Core is to provide a cost-effective fast method for generation and preservation of genetically altered mice for the research community.

We also have a variety of equipment that enhance the level of our services while further supporting your research projects. Let us show you how we can help you to conduct your transgenic and gene targeting research efficiently and cost-effectively. Contact us for a consultation, or see how to get started.