Single Cell Technology Core Equipment

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“10x

10x Genomics Chromium Connect

  • Robotic liquid handling system
  • Prepares up to eight samples simultaneously
  • Automates GEM generation, barcoding, and library preparation
  • Library options include 3’ gene expression or 5’ gene expression with V(D)J gene enrichment
  • Reduced interaction means minimal technical variation
“10x

10x Genomics Chromium Controller

 
 
 

The Chromium Controller is available for 10x experiments beyond the scope of transcriptomics. For a submitted tissue sample, individual cells or nuclei are barcoded, and libraries are prepared for RNA-Seq and ATAC-seq.

 
“10x

10x Genomics Chromium X

The Bioanalyzer is used to evaluate library integrity prior to any sequencing experiment to ensure the highest quality data is returned.

“1Agilent

Agilent 2100 Bioanalyzer

  • Maximizes future proofing
  • Scalable for evolving project needs and larger studies
  • Supports various applications including single-cell gene expression, whole exome-sequencing, and shotgun metagenomics
“NextSeq

NextSeq 2000

“NanoString

NanoString GeoMx Digital Spatial Profiler

State-of-the-art spatial biology is available with the NanoString GeoMx Digital Spatial Profiler. Given fresh frozen or FFPE tissue, this unique technology allows simultaneous, quantitative measurement of RNA and protein targets. The morphological context of the tissue can be resolved with customizable histology. The GeoMx assay is conducted by region of interest (ROI). There are several strategies for choosing the ROIs to maximize return on the experiment design. Choose between curated panels of molecular targets or customize your own. Instructions for panel selection are available here.

“Vizgen

Vizgen MERSCOPE

MERSCOPE is the first commercially available high-plex single-cell spatial genomics platform. Using MERFISH technology, transcripts from up to 500 genes can be mapped and quantified with single cell and subcellular resolution.

“10x

10x Genomics Visium CytAssist

10x Genomics Visium CytAssist allows investigators to map the entire transcriptome of H&E- or immunofluorescently stained tissue sections with morphological context. The innovative CytoAssist technology expands sample accessibility, enabling the transfer of transcriptomic analytes from tissue sections on standard glass slides into the Visium workflow.

“10x

10x Genomics Xenium

10x Genomics Xenium Analyzer provides easy to use, high throughput in situ profiling of hundreds of RNA targets at a subcellular resolution.

Akoya PhenoCycler-Fusion, through repetitive imaging cycles, enables the detection of a maximum of 40+ protein targets in the same tissue section at single-cell resolution, with quantitative data for each protein.

“Akoya

Akoya PhenoCycler-Fusion system