20 Llanfair Circle

Pediatric Gastroenterology and Hepatology
BIliary atresia
Cholestatic liver disease
Inflammatory bowel disease
Eosinophilic esophagitis

Serum miRNA as a biomarker

Research Interests
My laboratory conducts basic and translational research on microRNA in pediatric gastrointestinal and liver development and disease.

Liver development, microRNA, biliary atresia, cholestasis, fibrosis, inflammatory bowel disease, eosinophilic esophagitis.

Research details
1) MicroRNA in biliary atresia
Goal: to determine if microRNA plays a functional role in biliary atresia, using clinical samples, a mouse model of the disease, and cell culture tools.

2) MicroRNA in cholestatic liver disease
Goal: to understand how microRNA promotes the adaptive response to cholestasis.

3) MicroRNA in liver development
Goal: to determine if miRNA functions in liver development, particularly biliary specification and morphogenesis.

4) MicroRNA as a biomarker for gastrointestinal and liver disease.
Goal: to utilize circulating and tissue miRNA levels as diagnostic and prognostic biomarkers for diseases such as biliary atresia, inflammatory bowel disease, and eosinophilic esophagitis.

Rotation projects
MicroRNA target identification (several relevant miRNAs available)
Genetic models of specific miRNA deficiency
Functional analysis of miRNA in a cell culture model of biliary differentiation

Lab personnel
Nick Hand, Scientist
Adam Zahm, Post-doctoral Fellow
Claire Le Guen, Technician
Scott Gordon, MD/PhD student (post-PhD)

Assistant Professor of Pediatrics at University of Pennsylvania School of Medicine (2004 – 2013)
Associate Professor of Pediatrics at University of Pennsylvania School of Medicine (2013 – 2015)
Adjunct Associate Professor of Pediatrics at University of Pennsylvania School of Medicine (2015– present)
MD, University of Pennsylvania (1998)
PhD, Cell and Molecular Biology, University of Pennsylvania (1996)
AB, Biochemistry, Harvard University (1991)
Selected Publications
Kameswaran V, Bramswig NC, McKenna LB, Penn M, Schug J, Hand NJ, Chen Y, Won K-J, Vourekas A, Choi I, Liu C, Vivek K, Naji A, Friedman JR, Kaestner KH. Epigenetic regulation of the MEG3-DLK1 microRNA cluster in human Type 2 diabetic islets. Cell Metabolism. in press.
Le Guen CL, Friedman JR, Hand NJ. Novel targets of miR-30, a microRNA required for biliary development. F1000Research. Vol 2. 2013 October:197.
Karjoo S, Hand NJ, Loarca L, Russo PA, Friedman JR, Wells RG. Extra-hepatic cholangiocyte cilia are abnormal in biliary atresia. Journal of Pediatric Gastroenterology and Nutrition. Vol 57(1) . 2013 April:96-101.
Schug J, McKenna LB, Walton G, Hand N, Mukherjee S, Essuman K, Shi Z, Gao Y, Markley K, Nakagawa M, Kameswaran V, Vourekas A, Friedman JR, Kaestner KH, Greenbaum LE. Dynamic Recruitment of microRNAs to their mRNA Targets in the Regenerating liver. BMC Genomics. Vol 14(1) . 2013 April:264.
Zahm, AM, Hand, NJ, Boateng, LA, Friedman, JR. Circulating MicroRNA is a Biomarker of Biliary Atresia. Journal of Pediatric Gastroenterology and Nutrition. Vol 55(4) . 2012 October:366-9.
Venneti S, Boateng LA, Friedman JR, Baldwin DA, Tobias JW, Judkins AR, Mourelatos Z, Lal P. MiRNA-9 and miRNA-200a distinguish hemangioblastomas from metastatic clear cell renal cell carcinomas in the CNS.. Brain Pathology. Vol 22(4) . 2012 July:522-529.
Sonnenberg GF, Monticelli LA, Alenghat T, Fung TC, Hutnick NA, Kunisawa J, Shibata N, Grunberg S, Sinha R, Zahm AM, Sathaliyawala T, Kubota M, Farber DL, Collman RG, Shaked A, Fouser LA, Weiner DB, Tessier PA, Friedman JR, Kiyono H, Bushman FD, Chang K-M, Artis D. Innate lymphoid cells orchestrate anatomical containment of lymphoid-resident commensal bacteria and prevent systemic immune activation. Science. Vol 336(6086) . 2012 June:1321-1325.
Hand NJ, Horner AM, Master ZR, Boateng LA, LeGuen C, Uvaydova M, Friedman JR. MicroRNA profiling identifies miR-29 as a regulator of disease-associated pathways in experimental biliary atresia.. Journal of Pediatric Gastroenterology and Nutrition. Vol 54(2) . 2012 February:186-192.
Zahm AM, Thayu M, Hand NJ, Horner A, Leonard MB, Friedman JR. Circulating microRNA is a biomarker of pediatric Crohn disease. Journal of Pediatric Gastroenterology and Nutrition. Vol 53(1) . 2011 July:26-33.