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Hajime Takano, PhD
Hajime Takano
Research Assistant Professor of Neurology

Dr. Takano's research focuses on basic epilepsy and related neuroscience research centered on advanced optical imaging techniques such as fluorescence lifetime imaging and two-photon microscopy; and application of micro- and nanotechnology like graphene transparent electrode to functional cellular imaging.

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Bio

Dr. Takano’s research focuses on concepts related to neuroscience and engineering, with a particular interest in functional optical imaging to provide further knowledge on the basic mechanisms of epilepsy.

Dynamic Imaging of Nervous System Function: Dr. Takano specializes in characterization of the spatiotemporal firing patterns of multiple neurons using in vitro and in vivo multicellular calcium imaging by two-photon microscopy and laser scanning confocal microscopy. His expertise in analytical instrumentation enabled him to implement various imaging technologies such as fluorescence lifetime imaging, combined use of calcium imaging and voltage sensitive dye imaging, whole-field UV photolysis of caged compounds, optical fiber-based fluorescence imaging, and photon counting system for luminescence probes. More recently, he has implemented awake in vivo calcium imaging of mice navigating a virtual reality environment.

Micro- and Nanotechnology: Dr. Takano was originally trained in bioengineering/analytical chemistry laboratories and focused on the application of micro- and nanotechnology to chemical and biological analysis, especially using scanning probe microscopy. In 2014, Dr. Takano conducted a collaborative project aiming to prove that graphene, an atom-thick electrically conductive carbon sheet, could be used as a transparent electrode for neurosignal recording. The study was the first in the world to show that graphene can be used as a neurosignal recording electrode. In 2018, he obtained an NIH grant to study the cellular origin of high-frequency oscillations (HFOs), a potential biomarker for identifying the seizure onset zone, using graphene electrode array developed by a collaborator at Penn Engineering.

Training: As a core function of CHOP's Intellectual and Developmental Disabilities Research Center (IDDRC) and in a previous function at Penn, Dr. Takano has trained students and postdoctoral fellows in using advanced optical imaging techniques. Since 2005, he's trained and overseen more than 100 students and postdoctoral fellows from over 25 laboratories in the CHOP/Penn research community.

Education and Training

BS, Tokyo Institute of Technology (Biomolecular Engineering), 1992

MS, Tokyo Institute of Technology (Bioengineering), 1994

PhD, Tokyo Institute of Technology (Bioengineering), 1997

Postdoctoral Research Associate, Iowa State University (Analytical Chemistry), 1997-2000

Postdoctoral Research Associate, Iowa State University (Neuroscience), 2000-2001

Titles and Academic Titles

Research Assistant Professor of Neurology

Professional Memberships

American Chemical Society, 1997-

Society for Neuroscience, 2002-

American Epilepsy Society, 2018-

Publication Highlights

Dengler CG, Yue C, Takano H, Coulter DA. Massively augmented hippocampal dentate granule cell activation accompanies epilepsy development. Sci. Rep. 2017 Jan; 7: 42090. PubMed PMID: 28218241
Kuzum D*, Takano H*, Shim E, Reed JC, Juul H, Richardson AG, de Vries J, Bink H, Dichter M, Lucas TH, Coulter DA, Cubukcu E, Litt B. Transparent, flexible low-noise Graphene electrodes for simultaneous electrophysiology and neuroimaging. Nature Communications. 2014 Jan; 5: 5259. Online. PubMed PMID: 25327632 *first co-authors
Yu EP, Dengler CG, Frausto SF, Putt ME, Yue C, Takano H, Coulter DA. Protracted Postnatal Development of Sparse, Specific Dentate Granule Cell Activation in the Mouse Hippocampus. Journal of Neuroscience. 2013 Jan; 33(7): 2947-60. PubMed PMID: 23407953
Takano H, McCartney M, Ortinski PI, Yue C, Putt ME, and Coulter DA. Deterministic and Stochastic Neuronal Contributions to Distinct Synchronous CA3 Network Bursts. Journal of Neuroscience. 2012 Jan; 32(14): 4743-54. PMID: 22492030

Active Grants/Contracts

Dynamic Contributions of Identified Neurons to High Frequency

  • National Institute of Neurological Disorders and Stroke 
  • 2018-2020
  • The goal of this project is to develop transparent electrode array and apply the technology to cellular imaging study, especially to identify the cellular origin of high frequency oscillation in models of epilepsy.

Links of Interest