Metabolomic Core Services
- Consultation and Experimental Design
- Data analysis, interpretation and kinetic modeling
- Analytical Techniques
We offer a range of techniques to determine metabolomic profiling and fluxes, and accept samples obtained from in vivo studies, including human samples of blood, expired air, urine and biopsied tissues; and in vitro studies, such as tissue culture, and organ perfusion (we are unable to accept samples that contain radioactivity, infectious agents or biohazardous material).
Samples may be used for:
- analysis of isotopomers and isotopic enrichment (13C, 2H, 15N), to study metabolism of carbohydrates, amino acids, proteins, free fatty acids and lipids, in normal and disease states;
- measuring various metabolite levels;
- determining flux and metabolic rates; and
- testing new therapies for various diseases such as cancer, type 2 diabetes, inborn errors of metabolism, the metabolic syndrome, urea cycle defects, traumatic brain injury, and to determine the drug(s) impact on metabolic pathways as well as potential benefits and risks.
The MC@CHOP offers a variety of analytical techniques to quantitate stable isotope enrichment (13C, 2H, 15N) and to perform metabolic profiling. Our analytical repertoire includes the following:
Stable Isotope Analysis, including:
- Stable isotope enrichment (13C, 15N, 2H, 18O) in glucose, amino acids, NH3, urea, tricarboxylic acid cycle intermediates, fatty acids and other intermediates of metabolism
- 13CO2 enrichment in blood and/or expired air for studies of oxidative metabolism
- Doubly labelled water (2H2O/H218O ratio) for studies of metabolic rate in a freely moving organism
Measurement—both in vivo and in vitro—of flux through metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, fatty acid oxidation, the urea cycle, protein synthesis/degradation.
Metabolomics profiling including, measurements of amino acids, organic acids, NAD+, NADH, NADP+, NADPH, adenine nucleotides (AMP, ADP, ATP), Pi, triglycerides, free fatty acids, glucose, putrescine, pyridoxines, pyridoxal, protein, etc.
Carnitine/Acylcarnitines levels and profiling both in blood and tissue extracts
Measurement of neurotransmitters and neuromodulator levels and profiling
Measurement of D- or L- forms of amino acids and their levels both in physiological fluid and tissue extracts.
Measurement of selected drug, drug stability and/or drug metabolism as well as determining the impact of a given drug on metabolome and fluxome.
Measurement of signaling compounds such as cAMP in various tissues.