Human Pluripotent Stem Cell Core Services

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Below is a listing of the Human Pluripotent Stem Cell Core’s services and techniques.

iPSC lines

  • iPSC generation and iPSC characterization including authentication by DNA fingerprinting
  • Sequence confirmation of patient mutation
  • Stem cell gene expression panel
  • Pluripotency by in vitro differentiation
  • Karyotype analysis
  • CNV analysis
  • Mycoplasma testing
  • Clearance of reprogramming vectors
  • Surface stem cell markers

Gene editing of iPSCs

  • CRISPR-CAS9 gene editing to create a nucleotide change and/or a deletion
  • AAVS1 safe harbor targeting for transgene expression
  • Inducible gene expression, or rtTA gene targeting

iPSC in vitro differentiation

  • Generation of iPSC-derived hepatocytes, cardiomyocytes, megakaryocytes, erythrocytes, and/or macrophages
  • Generation of fresh or cryopreserved primitive hematopoietic progenitor cells and neuronal progenitor cells

Murine embryonic fibroblasts (MEFs)

  • Generation of cryopreserved CF1 low passage MEFs for expansion
  • Irradiated CF1 MEFs
  • Irradiated DR4 antibiotic resistant MEFs

Enrichment training
Hands-on, wet lab training for laboratory personnel for:

  • iPSC growth, maintenance, cryopreservation
  • Gene editing iPSC differentiation to blood or liver
  • PSC CRISPR-CAS9 gene editing
  • iPSC AAVS1 gene targeting

Micellaneous

  • iPSC cell sorting
  • Live culture of iPSC lines
  • Generating stocks of iPSC lines on MEFs or in feeder-free conditions
  • Generating iPSC cell pellet,
  • Monthly core usage charge

Reagents

  • Media
  • Supplements
  • Serum
  • Matrices
  • Small molecules
  • Cytokines