Flow Cytometry Equipment
For detailed information about our cell sorters and cytometers, please download the Instrument Optical Configuration PDF.
Accuri C6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to FACSCalibur. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts.
The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. End users with no previous flow cytometry experience can begin collecting data within minutes, with minimal help from the staff or following a quick-start guide. Detector voltages are fixed (cannot be changed by end user) and for many types of samples no settings need to be adjusted before recording data.
A full training session that covers the common features of Accuri C6, including compensation takes approximately one hour.
Accuri C6 links:
The Biosorter (Union Biometrica) is a jet-in-air sorter that can be used for separating large particles. Unlike FACSJazz and MoFlo Astrios which continuously generate droplets, the Biosorter relies on the air deflection of a continuous liquid stream into a tray.
It can be set up with two FOCAs (Fluidics and Optics Core Assemblies): 250 μm and 1,000 μm and it is equipped with two lasers and three fluorescence detectors.
The Biosorter can be used for purifying or enriching, large cells (megakaryocytes, adipocytes), cell clusters (pancreatic islets, Drosophila imaginal discs), whole organisms (C. elegans, zebrafish larvae) and various other particles (e.g. beads).
|Types of samples that can be sorted on the Biosorter||250 μm FOCA||1,000 μm FOCA|
|Large cells: adipocytes, hepatocytes, megakaryocytes, duct cells||Yes|
|Small cell clusters: stem cell spheres, mammospheres||Yes|
|Small organisms: C. elegans (embryo to adult), Drosophila (embryo to 1st instar)||Yes|
|Beads and other particles (20 - 150 μm)||Yes|
|Larger cell clusters: neurospheres, pancreatic islets, Drosophila imaginal discs.||Yes|
|Plants: Arabidopsis, tobacco (seeds, early seedlings)||Yes|
|Larger organisms: Drosophila (1st to 2nd instar), zebrafish larvae||Yes|
|Beads and other particles with diameter below 700 μm.||Yes|
We encourage end-users to learn how to operate the Biosorter and run their-own sorts. Typically one session of approximately 2 hours is sufficient for gaining basic sorting skills on Biosorter.
Researchers who prefer to have their samples sorted by the staff should contact us to discuss the project in detail.
CytoFLEX LX (6 lasers)
CytoFLEX LX has 6 lasers, 21 color detectors and the capability to detect microparticles as small as 200 nm by triggering off the side scatter detector. Instead of photomultipliers (PMTs) which are commonly used as detectors in most standard flow cytometers, CytoFlex relies on avalanche photodiode (APD) detectors which are characterized by excellent sensitivity and linearity. The characteristics of APDs make the compensation workflow very efficient and CytExpert software used on CytoFlex is easy to learn and operate.
The Cytoflex accommodates 12x75mm tubes, microtiter tubes and has a plate loader module that allows for automatic loading of samples from standard 96 well plates.
CytoFLEX S (4 lasers)
CytoFLEX S is the newest cytometer in our lab. It has 4 lasers, 13 color detectors and a plate loader module for running samples automatically, directly from standard 96 well plates. Note that in semi-automatic mode the CytoFLEX S can be used only with standard FACS tubes (12x75 mm); Eppendorf tubes cannot be used on CytoFLEX S at this time. The CytExpert software is intuitive and easy to learn.
This instrument is located in room ARC 1214 C.
Evos is a user-friendly inverted imaging system with the following features:
- Fully-automated digital system, fully controlled through software
- Touch-screen monitor
- Long working distance objectives: 1.25x, 4x, 10x, 20x, 40x
- Color camera for bright field and phase-contrast imaging
- Monochrome camera for fluorescence detection
- Up to four colors fluorescence detection. DAPI, GFP, and RFP light cubes are permanently installed. Cy5 light cube can be transferred from the Countess upon request.
- Motorized 3D stage
- Built-in software for scanning slides or plates, time-lapse imaging, Z stacks
Evos FL Auto Application Notes:
FACSAria fusion is located in room 1207H, it is equipped 4 lasers, 15 color detectors and it is operated using the FACSDiva software. Up to four populations can be sorted simultaneously on FACSAria and an Automatic Cell Deposition Unit (ACDU) can be used for sorting single or multiple cells in multiwell plates.
FACSCalibur is a cytometer with excellent sensitivity and provides flexibility to support a wide variety of research and clinical applications. It is a very good choice for experienced flow-ers.
FACSJazz is an electrostatic droplet sorter with blue, yellow-green and red lasers and 6 color detectors. It is encased in a class II biosafety cabinet and it is permanently fitted with 100 μm nozzle. The maximum cell sorting speed is up to 10,000 events/second (or 36 million events/hour) and maximum two populations can be sorted at a time. It has capabilities to sort in multiwell plates and to index sort.
Online reservations must be done at least one day in advance and all reservations for assisted use or full-service sorting have to be approved by the staff in order to become effective.
Users who need to sort cells on FACSJazz at least once a week are encouraged to learn how to use this instrument and take advantage of lower rates. Contact us for more details.
LSRFortessa is the most advanced cytometer in the lab. It is equipped with 5 lasers and 18 color detectors. It is operated using the FACSDiva software. In addition to the standard configuration shown above, LSRFortessa can be easily reconfigured to detect YFP and GFP in the same sample.
- FACSDiva quick reference guide
- Online course: BD FACSDiva™ Software v6 Overview
- LSRFortessa user manual
LSR II has been recently upgraded with a violet laser with 6 detectors and an additional detector off the red laser for Alexa fluor 700. LSR II has now 15 color detectors. LSR II and LSRFortessa are operated using the same software, FACSDiva.
MoFlo Astrios is a high speed cell sorter that can be outfitted with different nozzle sizes (70 and 100 μm are typical). With 4 lasers, 14 color detectors, and ability to detect particles as small as 200 nm using the forward scatter detector, Astrios is the most performant sorter in our lab. Maximum sorting speed with the 70 μm nozzle is 25,000 events/second (approx. 90 million events/hour); with 100 μm nozzle the maximum sorting speed is 10,000 events/second (or 36 million events/hour). Astrios is placed inside a class II biosafety cabinet and it can be used with samples that require containment up to BSL-2 .
Online reservations must be done at least one day in advance and all reservations need to be approved by the staff in order to become effective.
We encourage all end-users to learn how to monitor the sorts and operate the sorter in semi-assisted mode : the instrument is prepared by the staff (laser alignment, laser delays, drop delay etc.) and end users are responsible for feeding the sample and collection tubes into the sorter and monitoring the sort. The staff will be available to troubleshoot incidents and unexpected events.
Researchers interested to learn how to operate MoFlo Astrios independently should contact the staff. At least two training sessions of approximately 2 hours are at minimum required for acquiring the basic knowledge needed for starting up the sorter and setting up a cell sorting experiment. Additional hands-on practice is necessary for operating the sorter in a truly unassisted mode.