Gene Transfer
Recombinant viral vectors, single cell electroporation,
transgenic mice

Animals
Rats, mice, chicken embryos, Caenorhabditis elegans

In Vitro
Primary neuronal cell culture including cells isolated from the spinal cord (mixed cell and pure motor neuron), cerebellum and cerebral cortex, spinal cord explant cultures

Protein-Related
Immuno-histology, immuno-cytology, western blot, immune precipitation

Molecular Biology-Related
Cloning, PCR, site-directed mutagenesis