Serum miRNA as a biomarker
My laboratory conducts basic and translational research on microRNA in pediatric gastrointestinal and liver development and disease.
Liver development, microRNA, biliary atresia, cholestasis, fibrosis, inflammatory bowel disease, eosinophilic esophagitis.
1) MicroRNA in biliary atresia
Goal: to determine if microRNA plays a functional role in biliary atresia, using clinical samples, a mouse model of the disease, and cell culture tools.
2) MicroRNA in cholestatic liver disease
Goal: to understand how microRNA promotes the adaptive response to cholestasis.
3) MicroRNA in liver development
Goal: to determine if miRNA functions in liver development, particularly biliary specification and morphogenesis.
4) MicroRNA as a biomarker for gastrointestinal and liver disease.
Goal: to utilize circulating and tissue miRNA levels as diagnostic and prognostic biomarkers for diseases such as biliary atresia, inflammatory bowel disease, and eosinophilic esophagitis.
MicroRNA target identification (several relevant miRNAs available)
Genetic models of specific miRNA deficiency
Functional analysis of miRNA in a cell culture model of biliary differentiation
Nick Hand, Scientist
Adam Zahm, Post-doctoral Fellow
Claire Le Guen, Technician
Scott Gordon, MD/PhD student (post-PhD)
- Associate Professor of Pediatrics at University of Pennsylvania School of Medicine (2013– present)
- Assistant Professor of Pediatrics at University of Pennsylvania School of Medicine (2004 – 2013)
- MD, University of Pennsylvania (1998)
- PhD, Cell and Molecular Biology, University of Pennsylvania (1996)
- AB, Biochemistry, Harvard University (1991)
- Karjoo S, Hand NJ, Loarca L, Russo PA, Friedman JR, Wells RG. Extra-hepatic cholangiocyte cilia are abnormal in biliary atresia. Journal of Pediatric Gastroenterology and Nutrition. 2013 April.
- Schug J, McKenna LB, Walton G, Hand N, Mukherjee S, Essuman K, Shi Z, Gao Y, Markley K, Nakagawa M, Kameswaran V, Vourekas A, Friedman JR, Kaestner KH, Greenbaum LE. Dynamic Recruitment of microRNAs to their mRNA Targets in the Regenerating liver. BMC Genomics. Vol 14(1) . 2013 April:264.
- Zahm, AM, Hand, NJ, Boateng, LA, Friedman, JR. Circulating MicroRNA is a Biomarker of Biliary Atresia. Journal of Pediatric Gastroenterology and Nutrition. Vol 55(4) . 2012 October:366-9.
- Venneti S, Boateng LA, Friedman JR, Baldwin DA, Tobias JW, Judkins AR, Mourelatos Z, Lal P. MiRNA-9 and miRNA-200a distinguish hemangioblastomas from metastatic clear cell renal cell carcinomas in the CNS.. Brain Pathology. Vol 22(4) . 2012 July:522-529.
- Sonnenberg GF, Monticelli LA, Alenghat T, Fung TC, Hutnick NA, Kunisawa J, Shibata N, Grunberg S, Sinha R, Zahm AM, Sathaliyawala T, Kubota M, Farber DL, Collman RG, Shaked A, Fouser LA, Weiner DB, Tessier PA, Friedman JR, Kiyono H, Bushman FD, Chang K-M, Artis D. Innate lymphoid cells orchestrate anatomical containment of lymphoid-resident commensal bacteria and prevent systemic immune activation. Science. Vol 336(6086) . 2012 June:1321-1325.
- Hand NJ, Horner AM, Master ZR, Boateng LA, LeGuen C, Uvaydova M, Friedman JR. MicroRNA profiling identifies miR-29 as a regulator of disease-associated pathways in experimental biliary atresia.. Journal of Pediatric Gastroenterology and Nutrition. Vol 54(2) . 2012 February:186-192.
- Zahm AM, Thayu M, Hand NJ, Horner A, Leonard MB, Friedman JR. Circulating microRNA is a biomarker of pediatric Crohn disease. Journal of Pediatric Gastroenterology and Nutrition. Vol 53(1) . 2011 July:26-33.
- Laje P, Clark FH, Friedman JR, Flake AW. Increased susceptibility to liver damage from pneumoperitoneum in a murine model of biliary atresia. J Pediatr Surg. Vol 45(9) . 2010 Sep:1791-6.
- McKenna LB, Schug J, Vourekas A, McKenna JB, Bramswig N, Friedman JR, Kaestner KH. MicroRNAs Control Intestinal Epithelial Differentiation, Architecture, and Barrier Function. Gastroenterology. Vol 139(5) . 2010 Jul:1654-1664.
- Hand NJ, Master ZR, EauClaire SF, Weinblatt DE, Matthews RP, Friedman JR. The microRNA-30 family is required for vertebrate hepatobiliary development. Gastroenterology. Vol 136(3) . 2009 March:1081-1090. PMCID: PMC2672911.