Dr. Korchak's research focuses on positive and negative signal transduction for proinflammatory responses in human neutrophils such as secretion of oxygen radicals which is essential for microbicidal activity, but can also give rise to the tissue damage of inflammation in diseases such as sepsis, rheumatoid arthritis, and asthma.
Research in the laboratory is elucidating the positive and negative signalling mechanisms for phagocytic cell functions such as degranulation, release of toxic oxygen species, and apoptosis. We are investigating a family of structurally related, lipid-dependent protein kinases, protein kinase C, and their role(s) in signal transduction for oxygen radical generation and degranulation. We are defining how unique structural features of particular isotypes relates to their interaction with other signalling elements and to their specific roles in cell function(s). Antisense strategies are used to selectively deplete discrete PKC isotypes in order to evaluate their roles in signal transduction.
- Professor of Biochemistry and Biophysics at University of Pennsylvania School of Medicine (1992 – 2007)
- Research Professor of Pediatrics at University of Pennsylvania School of Medicine (1992 – 2007)
- Research Associate Professor of Pediatrics at University of Pennsylvania School of Medicine (1986 – 1992)
- Associate Professor of Biochemistry and Biophysics at University of Pennsylvania School of Medicine (1986 – 1994)
- Ph.D., Physiology, Tufts University (1962)
- Kilpatrick, L. E., Y.-H Song, M.W. Rossi and H.M. Korchak. Serine phosphorylation of p60 tumor necrosis factor receptor by protein kinase Cd in TNF-activated neutrophils. Amer. J. Physiol.. Vol 279. 2000:C2011-C2018.
- Yaney, G. C., H.M. Korchak and B.E. Corkey. Long chain acyl CoA regulation of protein kinase C and fatty acid potentiation of glucose-stimulated secretion in pancreatic b-cells (HIT). Endocrinology. Vol 141. 2000:1989-99.
- Korchak, M.M., M.W. Rossi and L.E. Kilpatrick. Selective role for b-PKC in signaling for O2 generation but not degranulation or adherence in differentiated HL cells. J. Biol. Chem.. Vol 273. 1998:27292-299.
- Deeney, J., B.A. Cunningham, S. Chheda, S., K. Bokvist, L. Juntti-Berggren, K. Lam, H.M. Korchak, B.E. Corkey and P.O. Berggren. Reversible Ca dependent translocation of protein kinase C and glucose-induced insulin release. J. Biol. Chem.. Vol 271. 1996:18154-18160.
- Kilpatrick, L.E., E. Jacabovics, L.J. McCawley, L.H. Kane and H.M. Korchak. Cromolyn inhibits assembly of the NADPH oxidase and superoxide anion generation by human neutrophils. J. Immunol. Vol 154. 1995:3429-3436.