epilepsy, hippocampus, GABA receptors, thalamus
Epilepsy, neuronal excitability, CNS rhythm generation, GABA receptors, development of neurotransmitter receptors and ion channels, synaptic function
Patch clamp recordings, extracellular and intracellular recordings, immunohistochemistry, molecular biology (including aRNA amplification mRNA expression profiling in individual neurons), neuronal cell culture, transgenic animals, in vitro and in vivo recording techniques, optical recordings, EEG recordings.
My research interests center on understanding the cellular and molecular mechanisms underlying the development of epilepsy. Symptomatic seizure disorders such as temporal lobe epilepsy are among the most prevalent and least medically responsive forms of epilepsy. They are also among the most interesting. A presumably normal individual receives some injurious stimuli, which, at some distant time point results in the initiation of an epileptic state, characterized by recurrent spontaneous seizures. A better understanding these seizure-initiating mechanisms should facilitate development of enhanced therapeutic strategies to improve treatment, and perhaps eventually contribute to the development of a cure for epilepsy.
My laboratory uses physiological, anatomical, and molecular techniques to address experimental issues relevant to epilepsy. Physiologically, my colleagues and I use patch clamp, intracellular, and extracellular recording techniques in both in vitro and in vivo preparations of animal or human brain. Anatomically, we use immunohistochemical and conventional staining techniques to characterize alterations occurring in the epileptic brain at a circuit level, including loss of populations of neurons, alterations in expression patterns of proteins, and axonal remodeling. Molecularly, we use a combination of semi-quantitative profiling of mRNA expression levels at the single cell level, in situ hybridization, retroviral transfection techniques, and antisense oligonucleotide knockdown of expression of certain proteins. The combination of these three diverse experimental approaches provides a powerful, synergistic approach to better understand critical factors contributing to the initiation of the epileptic condition.
- Professor of Neuroscience at University of Pennsylvania School of Medicine (2009– present)
- Associate Professor of Neuroscience at University of Pennsylvania School of Medicine (2008 – 2009)
- Professor of Pediatrics at University of Pennsylvania School of Medicine (2009– present)
- Research Associate Professor of Pediatrics at University of Pennsylvania School of Medicine (1999 – 2003)
- Associate Professor of Neuroscience at University of Pennsylvania School of Medicine (2001 – 2002)
- Associate Professor of Pediatrics at University of Pennsylvania School of Medicine (2003 – 2009)
- Ph.D., Biology, Boston University Marine Program, Marine Biological Laboratory, Woods Hole, MA (1986)
- M.S., Zoology, University of Rhode Island (1983)
- B.S., Biology with honors, Trinity College, Hartford, CT (1980)
- Cohen, A.S., Lin, D.D., Quirk, G.L. and Coulter, D.A.. Dentate granule cell GABAA receptors in epileptic hippocampus: enhanced synaptic efficacy and altered pharmacology. European Journal of Neuroscience. Vol 17. 2003:1607-1616.
- Burton, D.Y., Cunxian, S., Fishbein, I., Hazelwood, S., Li, Q., Defelice, S., Connolly, J.M., Perlstein, I., Coulter, D.A., and Levy, R.J.. The incorporation of an Ion channel gene mutation associated with the Long QT syndrome (Q9E-hMiRPI) in a plasmid vector for site-specific arrhythmia gene therapy: In Vitro and In Vivo feasibility studies. Human Gene Therapy. Vol 14. 2003:907-922.
- Rafiq, A., Gong, Q.Z., Lyeth, B.G., DeLorenzo, R.J., and Coulter, D.A.. Induction of prolonged electrographic seizures in vitro has a defined threshold and is all or none: implications for diagnosis of status epilepticus. Epilepsia. Vol 44(8) . 2003:1034-1041.
- Rikhter, T.Y., Hsu, F.C., and Coulter, D.A.. Gene expression alterations in CA1 area of the rat hippocampus before the onset of epilepsy: A microchip study. American Epilepsy Society. 2003 submitted.
- Ang, C.W., Carlson, G.C., and Coulter, D.A.. Stratum oriens/Alveus interneurons spatially segregate and gate excitatory inputs onto CA1 neurons: voltage sensitive dye imaging study. Society for Neuroscience Abstracts. 2003 submitted.
- Pathak, H.R., Kelly, M.E., Ackman, J., LoTurco, J.J., and Coulter, D.A.. Synaptic integration of newborn neurons in adult dentate gyrus following status epilepticus. Society for Neuroscience Abstracts. 2003 submitted.
- Crino, P., Jin, H., Rikhter, T.Y, Robinson, M.B., Coulter, D.A., and Brooks Kayal, A.R.. Increased expression of the neuronal glutamate transporter (EAAT3/EAAC1) in hippocampal and neocortical epilepsy. Epilepsia. Vol 43(3) . 2002:211-218.
- Gibbs III, J.W., Zhang, Y.-F., Ahmed, H.S., and Coulter, D.A.. Anticonvulsant actions of Lamotrigine on spontaneous thalamocortical rhythms. Epilepsia. Vol 43(4) . 2002:342-349.
- Itoh, T., Beesley, J., Itoh, A., Kavanaugh, B., Cohen, A.S., Coulter, D.A., Grinspan, J.B., and Pleasure, D.. AMPA glutamate receptor-mediated calcium signaling is transiently enhanced during development of oligodendrocytes. Journal of Neurochemistry. Vol 81. 2002:390-402.
- Coulter, D.A., McIntyre, D.C., and Loscher, W.. Animal models of limbic epilepsies: What can they tell us?. Brain Pathology. Vol 12. 2002:240-256.