Amy
 
R.
 
Brooks-Kayal
M.D.
Address: 
Abramson Pediatric Research Bldg. Division of Neurology- Room 502 3615 Civic Center Boulevard
215-590-2241
Affiliations
Expertise

Pediatric Epilepsy

Dr. Brooks-Kayal is interested in novel treatment strategies for pediatric epilepsy and proteomics, particularly the development of new techniques for sensitive identification of low abundance proteins that may bind to and regulate promoters of those genes that are thought to play a critical role in epileptogenesis.

KEY WORDS:
development, neurotransmitter receptors, GABA(A) receptors, GABA(B) receptors, NMDA receptors, AMPA receptors, GABA, glutamate, hippocampus, epilepsy

RESEARCH INTERESTS
Developmental and Molecular Neuroscience, Epilepsy

RESEARCH TECHNIQUES
molecular biology (incl. gene arrays, RT-PCR, western blotting), immunohistochemistry, in situ hybridization, CNS tissue culture and acute neuronal isolation, animal models of epilepsy, EEG, neurophysiology

RESEARCH SUMMARY
My research focuses on how developmental changes in excitatory & inhibitory neurotransmitter receptor subunit expression in hippocampal neurons impact on receptor function and pharmacology, on the effects of status epilepticus on the pattern of neurotransmitter receptor expression and on the role of these receptor changes in epileptogenesis in the developing and mature brain. We hypothesize that status epilepticus results in long-term disruptions in the pattern of excitatory & inhibitory neurotransmitter receptor expression in both the immature and adult brains which result in neuronal hyperexcitability and contribute to the process of seizure development and to the cognitive and behavioral co-morbidities of epilepsy. Additional areas of research interest investigation include the molecular signaling pathways that regulate neurotransmitter receptor expression changes after seizures and utilizing approaches to modify these alterations to determine if it impacts disease development.

Appointments
Associate Professor of Neurology at the Children's Hospital of Philadelphia (2005 – 2008)
Associate Professor of Pediatrics at University of Pennsylvania School of Medicine (2005 – 2008)
Assistant Professor of Pediatrics at University of Pennsylvania School of Medicine (1995 – 2005)
Assistant Professor of Neurology at the Children's Hospital of Philadelphia (1995 – 2005)
Education
M.D., Johns Hopkins University (1988)
B.A., Cornell University, College of Arts and Sciences (1984)
Selected Publications
Marsh E. and Brooks-Kayal, AR.. Status Epilepticus. The 5-Minute Pediatric Consultant, 4th Ed.,. Williams and Wilkins, Baltimore; 2005.
Marsh E. and Brooks-Kayal, AR.. Infantile Spasms. The 5-Minute Pediatric Consultant, 4th Ed.,. Williams and Wilkins, Baltimore; 2005.
Marsh E. and Brooks-Kayal, AR.. Seizures- Febrile. The 5-Minute Pediatric Consultant, 4th Ed.. Williams and Wilkins, Baltimore; 2005.
Marsh E. and Brooks-Kayal, AR.. Seizures- Partial and Generalized. The 5-Minute Pediatric Consultant, 4th Ed. Williams and Wilkins, Baltimore; 2005.
Marsh E. and Brooks-Kayal, AR.. Coma. The 5-Minute Pediatric Consultant, 4th Ed. Williams and Wilkins, Baltimore; 2005.
Raizen D. M. , Brooks-Kayal A.R., Steinkrauss L., Tennekoon G.I., Stanley C.A., and Kelly A.. Central nervous system hyperexcitability associated with glutamate dehydrogenase gain of function mutations. Pediatrics. Vol 146(3) . 2005 March:388-394.
Porter BE, Zhang G, Celix J, Hsu F-C, Raol YSH, Telfeian A, Gallagher PR, Coulter DA, Brooks-Kayal AR. Heterogeneous GABA(A) Receptor Subunit Expression in Pediatric Epilepsy Patients. Neurobiology of Disease. Vol 18(3) . 2005 April:484-489.
Raol YSH, Zhang G, Budreck EC, Brooks-Kayal AR. Long-term Effects of Diazepam and Phenobarbital Treatment during Development on GABA Receptors, Transporters and GAD.. Neuroscience. Vol 132(2) . 2005:399-407.
Zhang G., Y.H. Raol, F. Hsu, and Brooks-Kayal, AR. Long-term Alterations in Glutamate Receptor and Transporter Expression Following Early-Life Seizures are Associated With Increased Seizure Susceptibility.. J. Neurochem. Vol 88. 2004:91-101.
Zhang G, Raol, YH, Hsu FC, Coulter DA, Brooks-Kayal AR. Effects of Status Epilepticus on Hippocampal GABAA Receptors are Age-Dependent.. Neuroscience. Vol 125(2) . 2004:299-303.