The Flow Cytometry Core, located on the 12th floor of the Abramson Pediatric Research Building, room 1207, provides access to flow cytometry equipment and analysis software for trained personnel from individual research labs on a fee-for-service basis.
The core assists users in the design of experimental protocols, which require various flow cytometric methods. The laboratory is capable of both sample preparation and analyses in support of individual research efforts and offers specialized services such as training and mutiparametric cell sorting. The laboratory's current preparative and analytical capabilities include four-color immunophenotyping with software applications to support cell surface staining, intracellular staining (etc. intracellular cytokines, cellular proliferation, DNA analysis and apoptosis).
The laboratory performs flow-based Cytometric Bead Array assays for the simultaneous detection and quantitation of multiple cytokines in supernatants, plasma and serum. In addition, the core can assist in the preparation of technical literature and cost analyses as related to the grant application process.