Stocked Items

PCF Common Stocks

Chromatography Columns - Ciphergen Protein Chips - Plasmids and Transfer Vectors

The PCF routinely stocks commonly needed chromatography and desalting reagents. Additionally, we also stock various transfer vectors and plasmids for bacterial expression. Below is a partial listing of our available stock.

Chromatography Columns
HiTrap SP HP
HiTrap DEAE FF
HiTrap IEX Selection
HiTrap CM FF
HiTrap SP FF
HiTrap Q HP
HiTrap Chelating HP
NHS-activated sepharose 4 FF
HiTrap r Protein A
Source 15S
Source 15Q
CM Sepharose FF
DEAE Sepharose FF
SP Sepharose FF
Q Sepharose FF
GST Prep FF 16/10
Calmodulin Sepharose 4B
Streptaviding Sepharose HP
rProtein A Sepharose FF
HiPrep 26/10 Desalting
Glutathione Sepharose 4 FF
Mono Q 5/50 GL
Mono S HR 5/5
HiPrep 26/60 Sephacryl S-100HR
HiPrep 26/60 Sephacryl S-200HR
HiPrep 26/60 Sephacryl S-300HR
Superdex Peptide 10/300 GL
Mono P 5/200 GL
Superdex 200 10/300 GL

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Ciphergen Protein Chips
Gold Chip
SAX2 Chip
WCX2 Chip
PS10 Chip
PS20 Chip
Q10 Chip
IMAC3 Chip
IMAC30 Chip
H4 Chip
H50 Chip
CM10 Chip
NP20 Chip
RS100

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Vectors available at the PCF

Vector name
Features
Expression Host
Vendor
pENTR3C Gateway pENTR vector N/A Invitrogen
pENTR2B
Gateway pENTR vector N/A Invitrogen
pENTR1A Gateway pENTR vector N/A Invitrogen
pENTR4 Gateway pENTR vector N/A Invitrogen
pDONR221 Gateway pENTR vector N/A Invitrogen
pDEST10 Polyhedrin promotor, N-term 6XHis Baculovirus Invitrogen
pDEST15 N-term GST E. coli Invitrogen
pDEST17 N-term 6xHis E. coli Invitrogen
pDEST20 Polyhedrin promotor, N-term GST Baculovirus Invitrogen
pDEST42 C-term V5-6xHis E. coli Invitrogen
pAcGHLT-A transfer vector, N-term GST, 6xHis, thrombin cleavage site Baculovirus BD Biosicences
pAcGHLT-B transfer vector, N-term GST, 6xHis, thrombin cleavage site Baculovirus BD Biosicences
pAcGHLT-C transfer vector, N-term GST, 6xHis, thrombin cleveage site Baculovirus BD Biosicences
pAcUW51 transfer vector, Polyhedrin and p10 promotors, express 2 foreign genes Baculovirus BD Biosicences
pAcG3X transfer vector, N-term GST, factor Xa cleavage site Baculovirus BD Biosicences
pAcMp2 transfer vector, facilitates post-translational modifications Baculovirus BD Biosicences
pAcMP3 transfer vector, facilitates post-translational modifications Baculovirus BD Biosicences
pAcSecG2T transfer vector, polyhedrin promotor, signal sequence, GST-tag Baculovirus BD Biosicences
pFastBacHTa Invitrogen Bac-to-Bac Baculovirus system, N-term 6xHis , TEV cleavage site Baculovirus Invitrogen
pFastBacHTb Invitrogen Bac-to-Bac Baculovirus system, N-term 6xHis , TEV cleavage site Baculovirus Invitrogen
pFastBacHTc Invitrogen Bac-to-Bac Baculovirus system, N-term 6xHis , TEV cleavage site Baculovirus Invitrogen
BaculoDirect
C-term linear DNA
C-termV5-6XHis Baculovirus Invitrogen
pET-41b(+) GST, 6xHis, S tags, E. coli Novagen
pET-25b(+) C-term HSV and His, signal sequence, EK cleavage site, allow to have generation of native proteins with their authentic N-term E. coli Novagen
pET-32a(+) 6xHis, Trx, and S tags E. coli Novagen
pET-16b N-term 6xHis, Factor Xa cleavage site E. coli Novagen
pQE30Xa T5 promoter, N-term His tag, Factor Xa cleavage site E. coli Qiagen

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