Laser Microdissection and Pressure Catapulting (LMPC)

The PALM system from zeiss allows users to microdissect individual cells or regions of tissue from their slides for downstream molecular analysis.

Why Use Laser Dissection?

Heterogeneous tissue may confound molecular analysis because it is impossible to determine which cells contribute to which cellular constituent of a given lysate LMPC gives you the ability to isolate specific cells of interest without contamination from surrounding cells

The PALM Microbeam System

Specimen Requirements

Isolation of DNA

Beverly Emmanuel, CHOP
John Maris, CHOP

Isolation of RNA from Cryosections

Neil Rubenstein, UPENN

Isolation of Protein from specific cell populations

Carsten Bonneman, CHOP