1) High Resolution Spectroscopy
All classical 1D experiments are currently performed. These include:
- All types of high resolution spectra useful for solution studies.
- Variable temperature studies such as conformational analysis, spectroscopy of unstable or labile biomolecules.
- Quantitative analysis of complex mixture by 1H, 31P, 13C NMR spectroscopy. The minimum concentration measurable by proton or phosphorus NMR is of the order of 50 micromolar but highly dependent on experimental conditions.
- Structure identification of small and medium size organic molecules.
Many of the 2D experiments are also routinely performed. Theses include:
- Homo- and hetero-Correlation Spectroscopy (COSY, NOESY, HETCOR etc.). Cross peaks occurring in chemical shift correlation maps indicate either proton-proton (COSY) or proton-carbon connectivities (HETCOR) and are of invaluable use for spectral assignments.
- Spin Lock and Inverse experiments (ROSY, TOCSY, HSQC, HMQC, HMBC).
2) Magnetic Resonance Imaging
- The Micro 2.5 imaging probe is particularly well suited for imaging of specimens varying in size from 2 mm to 25mm, such as excised bone, artery, mouse lungs or fixed mice or rats embryos.
- The Mini 0.5 imaging probe can accommodate mice and small rats. The mouse cradle fits inside the 38mm RF coil and imaging of any part of the mouse body is feasible with or without cardiac or respiratory gating. For rats however, only the head can be imaged using the 38mm coil. Imaging of other parts of the body will require the use of a surface coil.
- $50/hr for CHOP users and $55/hr for outside users for work performed during the day (9:00am - 6:00pm)
- $25/hr for
work performed at night and during week ends.
New Client Information Form
In order to use the NMR Core, you must first fill out the appropriate registration form:
Internal CHOP Account Registration Form
External Acccount Registration Form
and fax the completed form to 215-590-3804, Attention: Lisa MacDowell.