Protocols and Links
Software Protocols and Links
SDS-7000 Software Operation Protocol.pdf
SDS-7500 Software Operation Protocol.pdf
SDS-7900HT Software Operation Protocol.pdf
On-line calendar signup protocol
Additional Useful Links
Real Time PCR experimental design workflow (still under construction)
RefSeq Genes exon-exon junction via UCSC Genome Browser
In-Silico PCR (ePCR) of Primer set via UCSC In-Silico PCR
TaqMan Chemistry and SYBR Green chemistry description
Primer Express Tutorial for Gene Expression Assay
Primer Express Tutorial for Allelic Discrimination Assay
Primers or probes selection criteria:
- Avoid runs of an identical nucleotide. This is especially true for guanine (G), where runs of four or more Gs should be avoided.
- Design primers as close as possible to the probe without overlapping the probe.
- Keep the G - C content within 30-80%.
- Select primers with a Tm of 58-60oC.
- The five nucleotides at the 3’ end should have no more than two G and/or C bases.
Real Time PCTR reagents selection helper
For Gene Expression Experiments that has very little starting materials, potential solutions – “Nugen’s WT-Ovation RNA Amplification System”
Universal Probe Library from Exiqon and Roche for assay design and synthesis.
All About Real Time PCR quick reference guide from Eurogentec