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Submit DNA template and primer in separate tubes (the Napcore provides standard primers).
For one sequencing rxn, submit the following amount of DNA template and primer in separate tubes (if 2 or more rxns are run on the same template, please double or multiple the amount of template DNA):
|DNA template||DNA Concentration||Sequencing primer|
|PCR product, 10 ul or more||10 ng/ul or higher||5 ul @ 2 uM|
|plasmids/phagemids/ M13phage etc., 10 ul or more||100 ng/ul||5 ul @ 2 uM|
|large insert clones, 10 ul or more
(size: 50 - 300 kb, cosmid, BACs, PACs etc)
price is different for such template
|200 ng/ul or higher||5 ul @ 6 uM|
It is our policy to rerun all reactions that don't work (this does not apply to samples that have low DNA concentration), this only applies to the same submission, it would be wise to submit twice as much DNA and primer as needed in case of rerun.
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