The premixed DNA sequencing request form

You have selected The premixed!
In order to save money you need to mix the DNA template and primer in a tube. (If the primer is a standard primer available from the Napcore, you don't have to add the primer)
For one sequencing rxn, mix the DNA and primer according to the table below:

DNA template DNA and primer
PCR product

mix 10 - 100 ng DNA and 2 picomole primer (1 ul of 2 uM) in a total of 9 ul. 10 ng per 100 bp.

i.e. for 400 bp product, 40 ng DNA is needed for sequencing.

100 ng is needed for fragments > 1 kb.

plasmids/phagemids/ M13phage etc. mix 200 ng DNA and 2 picomole primer (1ul of 2 uM) in a total of 9 ul
large insert clones (size: 50 - 300 kb) The premixed format cannot be used to submit large insert clones such as cosmid, BACs and PACs etc. Please use the traditional format to submit such template.

It is our policy to rerun all reactions that don't work the first time we run it, this only applies to the same submission, it would be wise to submit twice as much DNA and primer as needed in case of rerun. (18 ul instead of 9 ul.)


Step1: Fill in and Select "Submit" on page bottom

(Fields with an asterisk next to the name are required.)

P.I.'s Last Name:*
P.I.'s First Name:*
P.I.'s Middle Initial:
Submitter's Last Name:*
Submitter's First Name:*
Phone:*
Institution:*
P.O./Account No:*
Email address:*
Sample Type:* Sample Type can be PCR product, plasmid, phagemid, m13phage etc.
What data format you want:* Data format can be Mac, MS Windows, Linux, Unix etc. This is the data format of results we will send to you.

Please indicate if you want print out of the sequencing chromatogram. The default is no!

I don't need paper print out! I need paper print out!

If you have comments please type it here:

Please indicate how many rxn you want to submit:

I will enter my samples on the next page I have more than 35 samples, and will e-mail an Excel spreadsheet