You have selected The premixed!
In order to save money you need to mix the DNA template and primer in a tube. (If the primer is a standard primer available from the Napcore, you don't have to add the primer)
For one sequencing rxn, mix the DNA and primer according to the table below:
|DNA template||DNA and primer|
mix 10 - 100 ng DNA and 2 picomole primer (1 ul of 2 uM) in a total of 9 ul. 10 ng per 100 bp.
i.e. for 400 bp product, 40 ng DNA is needed for sequencing.
100 ng is needed for fragments > 1 kb.
|plasmids/phagemids/ M13phage etc.||mix 200 ng DNA and 2 picomole primer (1ul of 2 uM) in a total of 9 ul|
|large insert clones (size: 50 - 300 kb)||The premixed format cannot be used to submit large insert clones such as cosmid, BACs and PACs etc. Please use the traditional format to submit such template.|
It is our policy to rerun all reactions that don't work the first time we run it, this only applies to the same submission, it would be wise to submit twice as much DNA and primer as needed in case of rerun. (18 ul instead of 9 ul.)
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