METHODS AND ASSAYS OFFERED
The MC@CHOP offers a variety of analytical techniques to quantitate stable isotope enrichment (13C, 2H, 15N) and to perform metabolic profiling. Our analytical repertoire includes the following:
- Stable Isotope Analysis, including:
Stable isotope enrichment (13C, 15N, 2H, 18O) in glucose, amino acids, NH3, urea, tricarboxylic acid cycle intermediates, fatty acids and other intermediates of metabolism
13CO2 enrichment in blood and/or expired air for studies of oxidative metabolism
Doubly labelled water (2H2O/H218O ratio) for studies of metabolic rate in a freely moving organism
Measurement - both in vivo and in vitro - of flux through metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, fatty acid oxidation, the urea cycle, protein synthesis/degradation.
Metabolomics profiling including, measurements of amino acids, organic acids, NAD+, NADH, NADP+, NADPH, adenine nucleotides (AMP, ADP, ATP), Pi, triglycerides, free fatty acids, glucose, putrescine, pyridoxines, pyridoxal, protein, etc.
Carnitine / Acylcarnitines levels and profiling both in blood and tissue extracts
Measurement of neurotransmitters and neuromodulator levels and profiling
Measurment of D- or L- forms of amino acids and their levels both in physiological fluid and tissue extracts.
Measurment of selected drug and/or drug metabolites as well as determining the impact of a given drug on metabolome and fluxome.
Mesurement of signaling compounds such as, cAMP in various tissues.