Methods and Assays Offered
The MC@CHOP offers a variety of analytical techniques to quantitate stable isotope enrichment (13C, 2H, 15N) and to perform metabolic profiling. Our analytical repertoire includes the following:
- Stable Isotope Analysis
Stable isotope enrichment (13C, 15N, 2H, 18O) in glucose, amino acids, NH3, urea, tricarboxylic acid cycle intermediates, fatty acids and other intermediates of metabolism
13CO2 enrichment in blood and/or expired air for studies of oxidative metabolism
Doubly labelled water (2H2O/H218O ratio) for studies of metabolic rate in a freely moving organism
Isotopic enrichment is determined with either:
Liquid chromatography-tandem mass spectrometry
Gas chromatography-mass spectrometry
Isotope ratio mass spectrometry
Measurement – both in vivo and in vitro - of flux through metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, fatty acid oxidation, the urea cycle, protein synthesis/degradation.
Metabolomics profiling including, measurements of amino acids, organic acids, NAD+, NADH, NADP+, NADPH, adenine nucleotides (AMP, ADP, ATP), Pi, triglycerides, free fatty acids, glucose, protein, etc.
Carnitine / Acylcarnitines levels and profiling both in blood and tissue extracts
Measurement of neurotransmitters and neuromodulators levels and profiling, such as, epinephrine, norepinephrine, dopamine, octopamine or serotonin, both in physiological fluids (i.e., blood, CSF) and tissue extracts.
Measurment of D- or L- forms of amino acids and their levels both in physiological fluid and tissue extracts.
Measurment of selected drug and/or drug metabolites as well as determining the impact of a given drug on metabolomics and fluxomics.
Mesurement of signaling compounds such as, cAMP in various tissues.