GOALS AND RESEARCH FOCUS:
The Metabolomic Core at CHOP is a state-of-the-art research and analytical facility that supports researchers at CHOP and throughout the PENN campus. Our goal is to provide investigators with a resource that facilitates the analysis of major metabolic pathways in humans, animals and in vitro systems. Our analytical repertoire includes the following:
- The measurement - both in vivo and in vitro - of flux through major pathways of intermediary metabolism, including glycolysis, the tricarboxylic acid cycle, the oxidation of fatty acids and amino acids, the urea cycle and protein synthesis and degradation. Our methodology allows determination of metabolite turnover as well as the influence of regulatory factors in modulating flux.
- Determination of in vivo metabolic rate in freely moving organisms
- Determination of selected drugs and/or drug metabolites with triple stage quadrupole mass spectrometry
The diagram below exemplifies the kinds of analyses we perform. In this instance, we traced the metabolic fate of 13C-glucose and 15NH4Cl in a liver perfusion system in order to determine flux through glycolysis, the TCA cycle and the urea cycle (Biochem. J. 444: 537-51, 2012)